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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Nov 2016, 37 (6)                                     Back


nstantaneous and historical temperature effects on a-pinene

Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma Helianthus on male albino rat

 

 Mansour A. Al-Hazimi1, 2, Gomma M.N.2, Waggas A.S.2 and Sayed M. Rawi2*

1Biological sciences department, Faculty of Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

2Faculty of Sciences and Arts, Khulais, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

*Corresponding Author E-mail: rawisayed@yahoo.com

 

 

 

Publication Data

Paper received:

01 March 2016

 

Revised received:

26 May 2016

 

Re-revised received:

22 August 2016

 

Accepted:

22 August 2016

  

Abstract

 

Marine organisms represent a valuable source of new compounds. The present study focused on the neurotoxicity of ethanolic crude extract and partially purified protein of Anemone species collected from the Red Sea shore of Jeddah, Saudia Arabia. The ethanolic extracts were found toxic to rats and the median lethal dose (LD50) was 20.3 mg kg b. wt. after IP of the crude extract. The rats showed behavioral changes such as tonic convulsions, paralysis and flexing of muscles. A single dose equal to ? LD50 administered IP, the crude extract induced differential and time-dependentdopamine (DA), norepinephrine (NE), and serotonin (5-HT)neuronal neurotransmitter changes; reduced glutathione level(P< 0.01)and highly affected cerebral histopathological disturbances. The crude extract and peptide fraction of 1KDa showed highest norepinephrine (P <0.01) and dopamine (P<0.01) stimulatory activities. Whereas protein fraction of 500Da resulted in maximal increase (P <0.01) in serotonin post 30 and 60 min. In addition, the studied toxins vigorously affected rat brain cerebral cortex histological structure, the crude extract as well as the protein fraction of 1KDa disclosed the most histopathological effects. Light microscopy revealed neural hemorrhage, formation of pycnotic nuclei, marked edema due to enlargement of Virchow, congestion of blood vessels and focal hemorrhage. ??????????

 

 Key words

Gyrostoma helianthus, Neurotoxicity, Monoamines, Histopathology

 

 

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