Journal of Environmental Biology

Potential of Pseudomonas sp. JH 51-2 to stabilize lead in

mining site soil 

Jaehong Shim^1,2, Patrick J. Shea², Ik-Boo Jung¹, Byung-Taek Oh¹ and Min Cho¹*

¹Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea

²School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE 68583-0817, USA

*Corresponding Author?s E-mail: cho317@jbnu.ac.kr

 Publication Data

Paper received:

05 April 2014

Revised received:

05 December 2014

Re-revised received:

29 December 2014

Accepted:

25 February 2015

Abstract

The potential of a lead (Pb)-tolerant Pseudomonas sp. JH 51-2 strain to promote Pb stabilization was evaluated in mining site soil. The strain was isolated from soil containing multiple heavy metals. Transmission electron microscopy (TEM) showed that cell walls were surrounded by extracellular substances and scanning electron microscope-energy dispersive spectroscopy (SEM-EDS) confirmed the presence of Pb on the surface of cell membrane. Fourier transform infrared spectroscopy (FTIR) revealed that amino acids, phospholipids and extracellular polysaccharides were involved in Pb complexation and biosorption. Sequential extraction and toxicity characteristic leaching procedure (TCLP) showed decreased Pb availability in mining site soil in the presence of Pseudomonas sp. JH 51-2. Results indicate that Pseudomonas sp. JH 51-2 is an efficient biological stabilizer of Pb in contaminated soil. ?    

 Key words

Biostabilization, Lead, Pseudomonas sp., Sequential extraction, Toxicity characteristic leaching procedure

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