Journal of Environmental Biology

Development of in-vitro regeneration protocol for fast multiplication of guava (Psidium guajava L.) rootstock cv. Sardar

H. Singh¹, K. Kaur¹*, G. Kaur¹, G. Singh² and J.S. Brar¹      

¹Department of fruit science Punjab Agricultural University, Ludhiana-141 004, India

²Biotechnologist, School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141 004, India

Received: 23 May 2024                   Revised: 12 August 2024                   Accepted: 11 November 2024

*Corresponding Author Email : drkirankang@pau.edu                  *ORCiD: https://orcid.org/0009-0005-5375-2104

Abstract

Aim: To develop a systematic and robust in vitro multiplication method for production of guava rootstock.

Methodology: Nodal segments and shoot tip explants were sourced from in vitro and poly house raised seedlings. These explants were inoculated in Murashige and Skoog (MS) basal media supplemented with different concentrations and combinations of growth regulators for establishment, shoot proliferation and root induction.

Results: The supplementation of basal MS media with 6-Benzylaminopurine (BAP) (2.0 mg l^-1) + Kinetin (0.5 mg l^-1) facilitated the successful establishment of explants sourced from in vitro raised guava seedlings, resulting in higher establishment rates for nodal segments (62.67 %). Optimal shoot proliferation (55.70 %) and shoot length (2.78 cm) was achieved by incorporating BAP (2.0 mg l^-1) + gibberellic acid (GA₃) (0.5 mg l^-1) into the MS media whereas 1-Naphthaleneacetic acid (NAA) at a concentration of 0.5 mg l^-1 resulted in the highest levels of root induction (54.63%) and number of roots per shoot (2.94).

Interpretation: Successful in vitro regeneration of guava plants was attained by adding 2.0 mg l^-1 of BAP and 0.5 mg l^-1 of GA₃ to the MS medium for highest shoot proliferation with NAA @ 0.5 mg l^-1 for the optimum rooting of plantlets. The rooted plants were effectively acclimatized using a mixture of cocopeat, vermiculite and perlite (2:1:1) as a growing medium.

Key words: Micropropagation, Psidium guajava, Plant growth regulators, Tissue culture

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