Journal of Environmental Biology

Micronuclei and other nuclear abnormalities in phorate exposed fish, Channa punctatus

S.P. Trivedi¹*, A. Ratn¹, Y. Awasthi¹, N. Gupta², M. Kumar¹ and A. Trivedi³ 

¹Department of Zoology, Environmental Toxicology and Bioremediation Laboratory, University of Lucknow, Lucknow-226 007, India

²C.S.J.M. University, Kanpur-208 024, India

³Department of Animal Science, M.J.P. Rohilkhand University, Bareilly-243 006, India

*Corresponding Author Email : sat060523@gmail.com

Received: 26.10.2020                                                                   Revised: 12.03.32021                                            Accepted: 07.05.2021

Abstract

Aim: The present study aims to establish morphology-based nuclear abnormalities (NAs) including micronuclei (MN) as effective and sensitive genotoxic endpoint biomarkers in fish against the sub-lethal exposure of phorate.

Methodology: Fish, Channa punctatus (35 ± 3.0 g; 14.5 ± 1.0 cm) were randomly exposed in two sets, treated group 1 and 2 (0.0375 mg l^-1 and 0.075 mg l^-1 of phorate, respectively) along with a simultaneous control (0 mg l^-1). The blood was sampled after 30 days.

Results: A significant (p < 0.05) induction in reactive oxygen species (ROS) coupled with elevated frequency of blood cells showing micronuclei along with the gross appearance of notched nuclei, curved nuclei, blebbed nuclei, kidney-shaped nuclei, V-shaped nuclei, nuclear buds, nucleoplasmic bridges, dumbbell nuclei, and condensed/rounded nuclei were recorded in a dose-dependent manner.      

Interpretation: A significant (p < 0.05) induction in reactive oxygen species (ROS) coupled with elevated frequency of blood cells showing micronuclei along with the gross appearance of notched nuclei, curved nuclei, blebbed nuclei, kidney-shaped nuclei, V-shaped nuclei, nuclear buds, nucleoplasmic bridges, dumbbell nuclei, and condensed/rounded nuclei were recorded in a dose-dependent manner.

Key words: Channa punctatus, Micronuclei, Molecular biomarkers, Nuclear abnormalities, Oxidative stress, Phorate

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