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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Mar 2021, 42 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Assessment of genetic diversity and population structure in Indian mustard (Brassica juncea L.) using SSR markers

 

V.K. Singh, R. Avtar, Mahavir*, N. Kumari, Manjeet and R. Kumar 

Oilseeds Section, Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar,-125 004, India

*Corresponding Author Email : mahaveer.bishnoi9@gmail.com

 

 

Received: 13.06.2020                                                                   Revised: 15.11.2020                                                    Accepted: 15.12.2020

 

 

 

Abstract

Aim: Rapeseed-mustard is one of India's most popular oilseed crops, however, information on genetic diversity thereof is minimal. Better germplasm utilization for crop improvement can be achieved by enhanced understanding on this topic. Hence, population structure and genetic diversity of Indian mustard genotypes were studied using SSR markers.

Methodology: Ninety five diverse genotypes of B. juncea were procured from HAU, Hisar, IARI, New Delhi, DRMR, Bharatpur, and PAU, Ludhiana. Genomic DNA was extracted by modified CTAB protocol and quality was checked using agarose gel electrophoresis. A set of 70 random primers was used for DNA amplification. Data analysis was done by DARwin 6.0 and STRUCTURE v2.3.4 programs and dendrogram was generated using neighbour joining (NJ) method.

Results: Of 70 SSR markers, 44 were found to be polymorphic which amplified 157 alleles in 95 different genotypes with mean value of 3.57 alleles per locus and 0.48 of average polymorphic information content (PIC). The expected heterozygosity (He) and observed heterozygosity (Ho) values were 0.54 and 0.81, respectively. The primer BG 109 showed the highest effective multiplex ratio (EMR) value and marker index (MI) value, while BG 99 showed the highest discriminating power value (D). Jaccard's dissimilarity coefficient ranged from 0.137 to 0.77. Based on dissimilarity coefficient, M 13 and RC 47 were the most diverse genotypes (0.77).      

Interpretation: This research suggested a high genetic diversity among the genotypes of Indian mustard, which could be used in future crop improvement programmes for developing mustard cultivars and germplasm management practices.       

Key words: Genetic diversity, Indian mustard, Population structure, SSRs

 

 

 

 

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