Journal of Environmental Biology

Spermine induced protection of onion seed vigour and viability during accelerated ageing

Paper received: 13.11.2018??????? ??????????????????? Revised received: 12.01.2019???????? ??????????????????? Re-revised received: 22.01.2019???? ??????????????? Accepted: 28.01.2019

Authors Info

V.R. Yalamalle¹*, B.S. Tomar²,??? S.K. Jain³, A. Arora⁴, A. Kumar³ and A.D. Munshi² ?

¹ICAR-Directorate of Onion and Garlic Research, Pune-410 505, India

²Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India

³Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India

⁴Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India

*Corresponding Author Email :

vishwanath_yal@yahoo.com

Abstract

Aim: The aim of the present study was to investigate the role of spermine, a polyamine as a protective agent on accelerated ageing of onion seeds. ??

Methodology: Onion seeds variety Pusa Riddhi was primed with six concentrations of spermine (0.10 mM to 1.25 mM) and also with hydration and halopriming (2% K2HPO₄).? Hydrated, haloprimed and un-primed seeds were used as control. The primed and control seeds were accelerated aged at 45 ^oC and 100 % RH for 72 hr. Seed quality was assessed in control, freshly primed seeds, and in primed seeds subjected to accelerate ageing.

Results: All priming treatments enhanced the seed quality, there was 2.34?20.33 % increase in germination. Seed priming with 2% K₂HPO₄ had highest seed quality improvement which was at par with 1.25 mM spermine primed seeds. Enhanced seed vigour and the activity of antioxidant enzymes over un-primed seeds was observed in both 2% K₂HPO₄ and spermine primed seeds over unprimed seds. Seeds primed with 1.25 mM spermine recorded 66.66 % and 650 % increase in the activity of ROS scavenging enzymes SOD and POD respectively, but they were at par with halopriming. After accelerated ageing, deterioration in seed quality was minimal in seeds primed with spermine. Seeds primed with 1.25 mM spermine recorded 21.33% higher germination, 62.10 % higher speed of germination, 26.56 % longer seedlings, 13.68 % heavier seedlings and 175 % and 200 % higher SOD and POD activity as comparised to un-primed seeds. Seeds primed with 1.25 mM spermine also performed better over halopriming and hydropriming treatments.??     

Interpretation: Onion seed priming with 1.25 mM spermine was most effective treatment in enhancing the seed germination and vigour under accelerated ageing conditions.

Key words: Allium cepa, Halopriming, Seed priming, Seed vigour, Spermine

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