Journal of Environmental Biology

Heterotic grouping of late-maturing maize inbred lines based

on combining ability and molecular marker studies

Paper received: 10.05.2018??????????????????? Revised received: 30.10.2018????????????????? Re-revised received: 07.12.2018???????????? Accepted: 11.01.2019

Authors Info

R.T. Maruthi¹*, R.N. Gadag²,

J.S. Bhat³ and C.G. Karjagi⁴

¹ICAR-Central Research

Institute for Jute and Allied

Fibres, Barrackpore-700 120, India

²ICAR- Indian Agricultural Research Institute, New Delhi-110 012, India

³Regional Research Centre, ICAR-Indian Agricultural Research Institute, Dharwad-580 005, India

⁴ICAR-Indian Institute of Maize Research, Pusa Campus,

New Delhi-110 012, India

*Corresponding Author Email :

maru7806@gmail.com

Abstract

Aim: To examine concurrence between yield based and SSR marker based heterotic grouping of late-maturing maize inbred lines. ??  

Methodology: A total of 45 F1 crosses derived from 10x10 diallel mating scheme were evaluated along with their parents and hybrid checks across two locations (New Delhi and Dharawada). To group the inbred lines at molecular level, 35 polymorphic SSR primers were used for PCR amplification of repeat sequences from the genomic DNA of each inbred isolated by CTAB method. Cluster analysis was carried out by using NTSYS-pc- 2.02. 

Results: Analysis of variance revealed significant (P<0.01) variation among the parents and their hybrids across two locations for all the traits. The yield SCA classified 10 late-maturing maize inbred lines into three heterotic groups. Similarly, molecular genetic diversity analysis also categorized the inbred lines into three major clusters. However, correlations between SCA effects and genetic distance were low (r=0.161) for grain yield.

Interpretation: Heterosis prediction with the help of molecular markers alone was not found effective. Further, field-testing can be complimented with molecular markers for the elimination of inferior crosses.

Key words: Combining ability, Dendrogram, Heterotic groups, Maize, Simple sequence repeats

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