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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Jul 2019, 40 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Combined effect of zinc oxide nano particle incorporated chitosan

for better antimicrobial activity towards wound healing

 

Paper received: 10.05.2018??????????????????? Revised received: 26.10.2018????????????????? Re-revised received: 11.12.2018???????????? Accepted: 15.01.2019

 

 

Authors Info

S. Visnuvinayagam1*,

L.N. Murthy2, A. Jeyakumari2,

U. Parvathy3, R. Anandan4,

G.K. Sivaraman1 and

C.N. Ravishankar5

  

1MFB Division, ICAR- CIFT,

Cochin-682 029, India

 

2ICAR-Mumbai Research Centre of CIFT, Vashi- 400 703, India

 

3Fish Processing Division, ICAR- CIFT, Cochin-682 029, India

 

4Biochemistry and Nutrition Division, ICAR- CIFT, Cochin-682 029, India

 

5ICAR- CIFT, Cochin-682 029, India

 

    

 

*Corresponding Author Email :

visnuvinayagam@yahoo.co.in

 

 

Abstract

 

Aim: The aim of the present study was to characterize the zinc oxide nano particle incorporated Chitosan (ZnO-NP-CS) and its antimicrobial activity. ?  

 

Methodology: Zinc oxide nanoparticles (ZnO-NP) were prepared by sol-gel method and Minimum Inhibitory Concentration (MIC), Minimum bactericidal Concentration (MBC) and agar well diffusion method was used for the assessment of antibacterial activity of ZnO-NP and ZnO-NP-CS as well. 

 

Results: In UV?spectroscopy, blue shift in wavelength (~365 nm) corresponding to bulk ZnO particles (~385 nm) indicates the nano size. In SEM image, ZnO-NP appeared as nano flake shape and ZnO-NP treated Methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa (PA) bacteria illustrates leakage of intracellular content, fusion and shrinkage of bacteria, respectively. The MIC of ZnO-NP for most of food pathogens were between 0.01 to 0.1mg. Lower MIC was observed for Vibrio cholerae and Listeria monocytogenes; higher MIC was observed for Bacillus cereus and Pseudomonas aeruginosa. In antibiogram assay, the zone of inhibition of ZnO-NP-CS was equal to commercial antibiotics against Multiple Drug Resistant bacteria.

 

Interpretation: The combined effect of ZnO-NP and chitosan is better than the individual component, i.e., around 5?15 mm wider zone of inhibition than chitosan. ZnO-NP-CS can be a suitable alternative for the treatment of wound infected by multiple drug resistant bacteria.

 

Key words: Antibiogram, Chitosan, MRSA, Zinc oxide nanoparticles

  

 

 

 

 

 

 

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