JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Author Resources
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Jan 2018, 39 (1)                                     Back


nstantaneous and historical temperature effects on a-pinene

Gene expression profiling and expression analysis of freshwater shrimp (Neocaridina denticulata denticulata) using expressed sequence tags and short-term exposure to copper

 

N. Y. Kim1, E. J. Jeon1, S. H. Jung1, S. J. Ahn2, M. A. Park3 and J. S. Seo3*

1Pathology Research Division, National Institute of Fisheries Science (NIFS), Busan-46083, Korea

2Fisheries R&D Management Center, Korea Institute of Marine Science & Technology Promotion (KIMST), Seoul-06775, Korea

3Aquatic Disease Control Division, National Institute of Fisheries Science (NIFS), Busan-46083, Korea

*Corresponding Author E-mail: pharm001@korea.kr

 

 

 

Key words

Biomarker

Copper

Expressed sequence tags

Neocaridina denticulata

Transcriptome

 

 

 

Publication Data

Paper received : 28.11.2015?????????????????? Revised received : 23.05.2016????????????????????? Re-revised received : 30.06.2016 Accepted : 05.07.2016

 

Abstract

Aim: The aim of the current study was to investigate environmental potential biomonitoring marker to exposure the copperfrom freshwater shrimp using expressed sequence tag (EST) analysis.

 

Methodology: The freshwater shrimp was collected from Lake Chang Pyung, South Korea and made cDNA libraries from whole body. To analyze expression patterns of putative biomarkersaccording to the individuals'exposure to copper in a time-and concentration-dependentmanners, Quantitative PCR (qPCR) was conducted.??

 

Results: The expression levels of 9 potential biomarkers for heavy metal exposure including arginine kinase, acheron, cytochrome c oxidase subunit I, metallothionein, methylene tetrahydrofolatereductase, glutathione peroxidase, cathepsin L, cathepsin A and Cu/Zn superoxide dismutase genes were up-regulated 1-24 hr post-exposure to copper, in an exposure time- and/or dose-dependent manner. Furthermore, the discovery of genes that relate to heavy metal exposure and immune defense enables us to investigate the toxicity mechanisms and ecological effects of heavy metal pollution. ???

 

Interpretation: This study contributes to the identification of numerous EST clones that can be applied to further clarify the genetics and biomonitoring markers of freshwater shrimp.

 

 

Copyright ? 2018 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).