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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Jan 2017, 38 (1)                                     Back


nstantaneous and historical temperature effects on a-pinene

Detection of antimicrobial peptide genes from

antagonistic Bacillus subtilis (Bs_Ane) isolated from

Neil Islands of Andaman, India

 

K. Sakthivel1*, K. Manigundan1, R.K. Gautam1, I. Jaisankar1, Sushil K. Sharma2, Reena Singh1 and S. Dam Roy1

1Division of Field Crop Improvement and Protection, ICAR- Central Islands Agricultural Research Institute, Port Blair-744 101, India

2ICAR ? National Bureau of Agriculturally Important Microorganisms (ICAR-NBAIM), Mau Nath Bhanjan-275 001, India

*Corresponding Author E-mail: veluars@gmail.com

 

 

 

Key words

Andaman islands,

Antimicrobial peptides,

Bacillus subtilis,

Plant pathogens

 

  

Publication Data

Paper received : 22.12.2015

Revised received : 04.04.2016

Re-revised received : 24.06.2016

Accepted :? 13.07.2016

 

Abstract

Aim: The present study aimed to identify the native potential Bacillus strains of Neil Islands, which have biocontrol potential against major bacterial and fungal plant diseases of Andaman Islands.

 

Methodology: Twenty five Bacillus spp. isolated from chilli rhizosphere soils of Neil Island, South Andaman, India were characterized for antimicrobial potential by in vitro and glass house studies.? Further, the antimicrobial potential was confirmed by PCR amplification of AMP biosynthesis genes.

 

Results: Among 25 strains, Bacillus subtilis (Bs_Ane) strain showed better in vitro antagonistic potential (growth inhibition) against three? hytopathogens: Ralstonia solanacearum (15.2 mm), Xanthomonas oryzae pv. oryzae (29.3 mm) and Colletotrichum gleosporoides (48%). In glass house studies, Bs_Ane strain showed significant bio-control efficacy (88.9%) against chilli bacterial wilt disease. The strain identity of Bacillus subtilis was confirmed by 16s rRNA gene analysis and biolog based phenotypic fingerprinting. The antimicrobial potential of strain was further ascertained by the presence of eight antimicrobial peptide (AMP) gene biosynthesis in PCR amplifications, which confirmed the synthesis of five antibiotics (surfactin, bacilysin, subtilin, subtilosin and fengycins) by Bs_Ane strain.

 

Interpretation: The overall results revealed that Bacillus subtilis (Bs_Ane) strain from Neil islands could be used as a potential biocontrol agent either single/ in consortium while formulating broad spectrum plant disease management in Andaman Islands.

 

 

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