Journal of Environmental Biology

Chemical content and antimicrobial properties of three different extracts of Mentha pulegium leaves from Mugla Region, Turkey

 Nur Ceyhan-G?vensen¹* and Dilek Keskin²

¹Biology Department, Faculty of Science, Mugla Sitki Ko?man University, Mugla-48000, Turkey

²?ine Vocational High School, Adnan Menderes University, Aydin-09010, Turkey

*Corresponding Author E-mail: nurceyhan@msn.com

Publication Data

Paper received:

12 January 2016

Revised received:

25 March 2016

Re-revised received:

26 May 2016

Accepted:

02 June 2016

Abstract

The extract of ethanol, methanol and DMSO of pennyroyal leaves from Mugla Region (Turkey) were tested for antimicrobial activity against eleven bacterial and one yeast strain by disc diffusion method. Among the extracts assayed, the methanol extract of pennyroyal leaves exhibited signifiant antimicrobial activity against Salmonella typhimurium and Staphylococcus aureus with 20 mm diameter inhibition zone. The DMSO extract of pennyroyal leaves displayed significant activity against S.aureus (19 mm) and Bacillus subtilis (21mm)? showing inhibition zone of 19 mm and 21 mm diameter, while the ethanolic extract showed significant antimicrobial activity against S.aureus (17mm) and Klebsiella pneumoniae (20mm) inhibition zones.? On comparint the MIC value of ethanol, methanol and DMSO leaf extract, methanolic extract of pennyroyal presented best activity (MIC 8 mg ml-1) against S. typhimurium CCM 583 and S.aureus ATCC 6538/P. Analyses of GC/MS determined eleven compounds viz., neophytadiene (69.95%), Pulejon 7.85%, Pinane 4.81%, Bicyclo (3.1.1 Heptane 2.6.6.6 trimethyl) 4.68%. In?? conclusion, methanolic extracts of M. pulegium showed antimicrobial activity because of high neophytadiene content.?????????

 Key words

Antimicrobial activity, Inhibition zone, Mentha pulegium

Copyright ? 2016 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).