Journal of Environmental Biology

Occurrence and characterization of hitherto unknown

Streptomyces species in semi-arid soils

Surendra Kumar¹, E. Priya¹, Dilip Singh Solanki¹, Ruchika Sharma¹, Praveen Gehlot¹*, Rakesh Pathak²? and S.K.? Singh²

¹Mycology and Microbiology Laboratory, Department of Botany, JNV University, Jodhpur-342 001, India

²Central Arid Zone Research Institute, Jodhpur- 342 003, India

*Corresponding Author E-mail: drpg73@rediffmail.com

Publication Data

Paper received:

29 April 2015

Revised received:

11 February 2016

Re-revised received:

05 March 2016

Accepted:

19 March 2016

Abstract

Streptomyces the predominant genus of Actinobacteria and plays an important role in the recycling of soil organic matter and production of important secondary metabolites. The occurrence and diversity assessment of Streptomyces species revealed alkaline and poor nutrient status of soils of semi-arid region of Jodhpur, Rajasthan. The morphological and biochemical characterization of 21 Streptomyces isolates facilitated Genus level identification but were insufficient to designate species. Species designation based on 16S rRNA gene delineated 21 isolates into 14 Streptomyces species. Upon BLAST search, the test isolates exhibited 98 to 100% identities with that of the best aligned sequences of the NCBI database. The GC content of 16S rRNA gene sequences of all the Streptomyces isolates tested ranged from 59.03% to 60.94%. The multiple sequence alignment of all the 21 Streptomyces isolates generated a phylogram with high bootstrap values indicating reliable grouping of isolates based on nucleotide sequence variations by way of insertion, deletion and substitutions and 16S rRNA length polymorphism. Some of the Streptomyces species molecularly identified under present study are reported for the first time from semi-arid region of Jodhpur.

Key words

16SrRNA gene, Diversity, GC content, Streptomyces

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