Journal of Environmental Biology

Physiological properties of Scomber japonicus meat hydrolysate prepared by subcritical water hydrolysis

Jae-Suk Choi^1,2, Hye Eun Moon¹, Myong-Kyun Roh³, Yu-Mi Ha¹, Bo-Bae Lee¹,

Kwang Keun Cho⁴ and In Soon Choi^1,5*

¹RIS Center, Industry-Academic Cooperation Foundation, Silla University, Busan 617-736, Republic of Korea

²Division of Bioindustry, College of Medical and Life Science, Silla University, Busan 617-736, Republic of Korea

³Department of R&D, Korea Bio & Engineering. Co., Ltd, Nam-gu, Busan 608-736, Republic of Korea

⁴Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, 150 Chilam-dong,

Jinju, Gyeongnam, 660-758, Republic of Korea

⁵Department of Biological Science, Silla University, Busan 617-736, Republic of Korea

*Corresponding Author E-mail : ischoi@silla.ac.kr

Publication Data

Paper received:

23 July 2013

Revised received:

01 January 2014

Accepted:

03 March 2014

Abstract

The health-beneficial biological activities, including antioxidant and tyrosinase inhibitory activities, of Scomber japonicus muscle protein hydrolysates prepared by subcritical water hydrolysis were investigated. After 5 min of subcritical hydrolysis at 140?C, 59.76% of S. japonicus muscle protein was hydrolyzed, the highest degree of hydrolysis in all the groups were tested. According to the response surface methodology results, as the reaction temperature and reaction time became lower and shorter, the yield became higher. The highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity (90.63%) occurred in hydrolysates treated at 140?C for 5 min, and the highest tyrosinase inhibitory activity (65.54%) was identified in hydrolysates treated at 200?C for 15 min. Changes in the molecular weight distribution of S. japonicus muscle proteins after subcritical water hydrolysis were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Subcritical water hydrolysis is a suitable technique for obtaining S. japonicus muscle protein hydrolysates with useful biological activities, within a short time (5?15 min).   

 Key words

Antioxidant activity, Hydrolysate, Scomber japonicus, Subcritical water hydrolysis, Tyrosinase inhibitory activity

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