Journal of Environmental Biology

Generation of cytotoxic molecules and oxidative stress in haemolymph of pebrinised tasar silkworm Antheraea mylitta Drury

Karmabeer Jena¹*, Jay Prakash Pandey¹, Anshu Priya², Papri Kundu¹, Ajit Kumar Sinha¹,

Harendra Yadav¹ and Alok Sahay¹

¹Central Tasar Research and Training Institute (Central Silk Board), Piska-Nagri, Ranchi-835 303, India

²St.Xavier's College, Dr.Camil Bulke Path, Pathalkudwa, Nayatoli, Ranchi-834 001, India

*Corresponding Author E-mail: jenakb2010@gmail.com

Publication Data

Paper received:

28 January 2015

Revised received:

14 August 2015

Re-revised received:

23 October 2015

Accepted:

09 October 2015

Abstract

The present study was carried out to investigate the effects of microsporidial infection on redox regulation mechanism and oxidative stress in tasar silkworm Antheraea mylitta. High level of superoxide radical (p < 0.05), nitric oxide (p < 0.001) and lipid peroxidation (p < 0.001) was observed in haemolymph of pebrinised larvae, which indicated the resultant generation of cytotoxic molecules and oxidative damage. Increased phenol oxidase (PO) activity in haemolymph of pebrinised larvae indicated the activation of immune defences during pathological conditions. In addition, higher level of glutathione-S-tranferase (GST) activity and reduced glutathione (GSH) level observed in pebrinised larvae indicated adaptive behaviour of tissue against toxic oxyradicals (p < 0.05). Conversely, low level of ascorbic acid (ASA) content suggested that the larvae might have used these compounds to counteract stress in tissues or low uptake under microspridial infection (p < 0.05). Present findings provide new insights into the cellular and biochemical bases of host-pathogen interactions in tasar silkworm A. mylitta.   

 Key words

Antioxidants, Cytotoxic molecules, Oxidative stress, Tasar silkworm 

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