Journal of Environmental Biology

Effects of root exudates of bivalent transgenic cotton (Bt+CpTI) plants on antioxidant proteins and growth of conventional cotton (Xinluhan 33)

Hong-sheng Wu¹*, Xue Shi¹, Ji Li¹, Tian-yu Wu³, Qian-qi Ren¹, Zhen-hua Zhang², Ming-yan Wang¹,

Xiao-xia Shang¹, Yan Liu²? and Song-hua Xiao⁴*

¹Department of Agricultural Resources and Environment, Nanjing University of Information Science and Technology, Nanjing 210044, China

²Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing 210012, China

³Department of Biotechnology, Wuhan Institute of Bioengnieering, Wuhan 430415, China

⁴Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

*Corresponding Author E-mail : wuhsglobe@sina.com, njxsh@sina.com

Publication Data

Paper received:

08 August 2014

Revised received:

05 January 2015

Accepted:

20 March 2015

Abstract

A greenhouse experiment was conducted to assess the adverse impact of transgenic cotton on ecosystem and environment via effect of transgenic Bt+CpTI cotton root exudates on growth and antioxidant activity of conventional parental cotton. Results showed elevated reductive and oxidative species activities in the leaves of conventional parental cotton seedlings treated with varying concentrations of transgenic cotton root exudates. Compared to control, 14.9% to 39.9% increase in catalase, 8.8% to 114% increase in for peroxidase, 21.3% to 59.7% increase in phenylalanine ammonia-lyase and 5.8 to 19.5 fold in ascorbate specific peroxidase was observed. However, biomass and height of conventional cotton seedlings were not affected by any concentration of transgenic cotton root exudates. These results suggested that cultivation of transgenic Bt+CpTI cotton plants poses little risk to conventional parental cotton based on their root interactions.   

 Key words

Antioxidase activity, Risk and biosafety assessment, Root exudates, Transgenic cotton

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