Journal of Environmental Biology

In vitro studies on anti-obesity activity of Korean Memilmuk through AMPK activation

Gil-Sun Park¹, Yu-Mi Jeon¹, Ji-Hye Kim¹, Sang-Kyu Park² and Mi-Young Lee^1,2*

¹Department of Medical Science, Graduate School of Soonchunhyang University, Asan, Chungnam 336-745, Republic of Korea

²Department of Medical Biotechnology, Soonchunhyang University, Asan, Chungnam 336-745, Republic of Korea

*Corresponding Author E-mail: miyoung@sch.ac.kr

 Publication Data

Paper received:

11 March 2015

Revised received:

30 July 2015

Re-revised received:

14 October 2015

Accepted:

03 November 2015

Abstract

The anti-obesity effect of Korean traditional food, Memilmuk, was examined through inhibition of differentiation of 3T3-L1preadipocytes by buckwheat flour extract. Oil-Red O staining showed that lipid accumulation in adipocytes was reduced upon adding buckwheat flour extract, indicating effective inhibition of adipocyte differentiation. Buckwheat flour extract also inhibited the expression of adipogenic transcription factor, peroxisome proliferator-activated receptor g (PPARg), and AMP-activated protein kinase (AMPK), an intracellular regulator of energy balance. Overall, the anti-obesity effect of Korean Memilmuk might be mediated through down-regulation of PPARg expression via AMPK activation by buckwheat flour.   

 Key words

3T3-L1 cells, AMPK, Anti-obesity, Memilmuk

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