Journal of Environmental Biology

Population distribution and genetic relatedness in

Indian Fusarium udum isolates based on ribosomal internal

transcribe spacer and elongation factor

Khela R. Soren¹*, Priyanka Gangwar¹, R.G. Chaudhary² and Subhojit Datta¹

¹Biotechnology Unit, Indian Institute of Pulses Research, Kanpur-208 024, India

²Plant Pathology Division, Indian Institute of Pulses Research, Kanpur-208 024, India

*Corresponding Author E-mail: sorenars@gmail.com

Publication Data

Paper received:

02 April 2014

Revised received:

19 August 2014

Accepted:

18 October 2014

Abstract

With the objective to study the geographical distribution pattern and pathotype classification, isolates from 12 major pigeonpea growing states of India were examined at morphological and molecular levels. Two DNA based internal transcribe spacer (ITS) region derived primers FDP 3 (ITS1/ITS2), FDP 25 (mRNA, LOC100383610) and two elongation factors FDP 4 (F98-BKR5) and FDP 29 (M9968PY) were employed to genetically differentiate the isolates. As a result, each marker system gave an average of 3 alleles/marker. The higher efficiency of ITS over EF-1α marker was revealed using detailed comparative analysis that included various parameters like gene diversity index, effective marker ratio, and marker index. Neighbour Joining tree analysis grouped the isolates into three major clusters and showed narrow existence of genetic divergence. Combination of genotyping data with pathological measurements indicates dominance of variant 1 in the Central zone, South zone and North East Plain Zone, while North East Plain Zone and North West Plain Zone were largely dominated by variants 2 and 1, with strong possibility of evolving other variants. The present study would help in identifying specific isolate and patterns of its distribution in various pigeonpea growing regions thereby enhancing the scope for precise resistance breeding for crop improvement.   

 Key words

Elongation factor, Fusarium udum, Genetic diversity, Internal transcribed spacer, Pathogenicity

Copyright ? 2015 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).