Journal of Environmental Biology

Assessment of genetic diversity in quality protein maize(QPM) inbreds using ISSR markers

Devraj Lenka¹, Swapan K.Tripathy¹*,Ramesh Kumar², Meenakshi Behera¹ and Rajesh Ranjan¹

¹Department of Plant Breeding and Genetics, College of Agriculture, OUAT, Bhubaneswar-751 003, India

²Directorate of Maize Research, New Delhi-110112, India

*Corresponding Author?s Email : swapankumartripathy@gmail.com

 Publication Data

Paper received:

26 December 2014

Revised received:

28 May 2014

Accepted:

28 September 2014

Abstract

Genetic diversity of 49 maize inbreds was assessed using twelve ISSR primers. A wide variation in PCR products was revealed in terms of size (280 to 3000bp), extent of polymorphism (94.87%) and number of bands (4-9). As a whole, 78 ISSR bands were produced (including four monomorphic bands) with an average of 6.5 bands per primer and the maximum number of bands (9) being produced by primer OUAT-8. Five ISSR primers (OUAT-8, OUAT-9, OUAT-15, OUAT 17 and OUAT-18) revealed higher PIC value (around 0.70) along with 100% polymorphism indicating better allelic diversity. While, ISSR primer OUAT-15 revealed higher number of polymorphic bands (8) with 100% polymorphism as well as considerably high PIC and Rp values. Thus, such an informative and discriminative primer is of immense value for the study of genetic diversity in a set of maize genotypes. The similarity index values ranged from 0.3 to 0.9 with an average of 0.522 and BQPM-1-14 maintained the highest genetic distance as revealed from its lowest average similarity coefficient value (0.393) with rest of the genotypes.? BQPM 1-14, BQPM 1-8, BQPM 3-10, BQPM 6-8 and B 1110-7-2 were identified to be highly divergent among the test inbreds which could be sorted out as valuable materials for heterosis breeding for production of single cross hybrids.     

 Key words

Genetic diversity, Quality protein maize inbreds, ISSR markers 

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