Journal of Environmental Biology

Biodecolourisation of reactive red an industrial dye by Phlebia spp.

Mukesh Chander¹*, Daljit Singh2 and Ramandeep Kaur²

¹Bioprocess Laboratory, P.G. Department of Biotechnology, Khalsa Coll?ge, Amritsar?143 002, India

²Department of Microbiology, G.N.D. University, Amritsar?143 005, India

*Corresponding Authors Email : mukeshchander76@yahoo.co.in

Publication Data

Paper received:

21 January 2013

Revised received:

16 August 2013

Re-revised received:

17 September 2013

Accepted:

05 October 2013

Abstract

Four white rot fungi namely Phanerochaete chrysosporium, Phlebia floridensis, P. radiata and P. brevispora were selected for their ligninolytic enzymes viz., lignin peroxidase, manganese peroxidase and laccase. Cell free enzyme extracts (as such and concentrated) obtained from these fungi were tested for their ability to decolourise reactive red 28 (Congo red), an industrial dye. The use of cell free enzyme extracts helped to overcome the problem of adsorption of dye to mycelia. Laccase production was best expressed in P. brevispora. Increase in decolourisation percentage by concentrated culture extract was comparable to increase in enzyme activity. P. floridensis proved to be a better dye decolouriser in comparison to Pha. chrysosporium, thus showing its potential for biocleaning of industrial wastes and wastewaters.

Key words

Congo red, Decolourisation, Enzyme extract, Fungi, Ligninolytic enzymes 

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