Journal of Environmental Biology

Effects of tert-butyl hydroperoxide on Ca²⁺ ATPase activity in isolated

rat hepatocytes and its reversal by antioxidants

Author Details

Sangram Singh

(Corresponding author)

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India ?????????????????

e-mail : sangram_rml@yahoo.co.in

Richa Agarwal

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India

Farrukh Jamal

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India

Sudhir Mehrotra

Department of Biochemistry, University of Lucknow , Lucknow-226007, India ^?????

Rakesh Singh

Department of Biochemistry, Banaras Hindu University , Varanasi-221104, India

Publication Data

Paper received:

27 November 2010

Revised received:

16 May 2011

Accepted:

11 June 2011

Abstract

Calcium ions play an important role in various physiological processes such as nerve impulse transmission, muscle contraction, hormone action, blood clotting. They ions act as an intracellular second messenger, relaying information within cells to regulate their activity. To understand the mechanism of hepatotoxicity of t-BHP, studies were carried out using freshly isolated rat hepatocytes. The effect of t-BHP on Ca²⁺ accumulation and Ca²⁺ uptake by rat hepatocytes was monitored using ⁴⁵Ca²⁺. It caused decrease in 15% accumulation of ⁴⁵Ca²⁺ in comparison to the control group. t-BHP also significantly decreased the Ca²⁺ ATPase activity in isolated hepatocytes. This decrease in Ca²⁺ATPase activity by t-BHP was reversed 40% by naturally occurring antioxidant glutathione (GSH) and 20% by the synthetic antioxidant butylated hydroxy toluene (BHT). These results indicate that the hepatotoxic action of t- BHP involves oxidative stress as evident by the protection accorded by various antioxidants employed in the study as well as impairment of intracellular calcium homeostasis which can lead to liver cell injury.

Key words

Oxidative stress, Reactive oxygen species, Hepatotoxicity, Tert-butyl hydroperoxide, Calcium homeostasis.

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