Journal of Environmental Biology

Studies on strains of Trichoderma spp. from high altitude of    

Garhwal Himalayan region

 

Author Details

 

B. B.  Joshi

(Corresponding author)

Indian  Institute  of  Sugarcane  Research,  Lucknow-226002, India

e-mail :  bjoshi92@yahoo.com

M. P. Vishwakarma

Department  of  Botany,  HNB Garhwal  University,  Srinagar, Garhwal-246174, India

D. Bahukhandi

Division of Seed Technology, Indian Grassland and Fodder Research Institute, Jhansi-284003, India

R. P. Bhatt

Department  of  Botany,  HNB Garhwal  University,  Srinagar, Garhwal-246174, India

 

 

Publication Data

Paper received:

08 February 2010

 

Revised received:

06 September 2011

 

Accepted:

18 October 2011

 

Abstract

Two species of Trichoderma i.e. T. harzianum and T. viride have been isolated from the soil samples collected from the higher altitude (2000-3500 m) of Garhwal Himalayan region in India. The two species were grown in Petri plates on TSM agar media and it was also observed that the optimum temperature and pH for Trichoderma growth was 30⁰ C and 5.5 respectively. When incubated on TSM agar medium at 4⁰ C, the fungus grew normally with heavy induced sporulation within three weeks of incubation. Induction of sporulation on exposure to low temperature appeared to be strategies for survival of these species in extreme cold environment temperature 4 to 5⁰ C. Antifungal activities of the two species of Trichoderma were demonstrated with phytopathogenic fungi in dual cultures. The antifungal metabolites produced by Trichoderma spp., diffusible as well as volatile, caused abnormalities in pathogenic fungi. Plant growth promotion of Trichoderma spp. was also shown through plant analysis in greenhouse. 

 

Key words

Trichoderma spp., Trichoderma selective medium, Biocontrol agent, Soil-borne pathogens

 

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