Journal of Environmental Biology

Morphometric and molecular characterization of fungus

Pestalotiopsis using nuclear ribosomal DNA analysis

Author Details

Praveen Gehlot

(Corresponding author)

Department of Botany, JNV University, Jodhpur - 342 001, India?

e-mail: drpg73@rediffmail.com

S.K. Singh

Central Arid Zone Research Institute, Jodhpur - 342 003, India

Rakesh Pathak

Central Arid Zone Research Institute, Jodhpur - 342003, India

Publication Data

Paper received:

28 January 2011

Revised received:

05 May 2011

Accepted:

25 May 2011

Abstract

Taxonomy of the fungus Pestalotiopsis based on morphological characters has been equivocal. Molecular characterization of ten Pestalotiopsis species was done based on nuclear ribosomal DNA internal transcribed spacer (ITS) amplifications. Results of the analyses showed that species of genus Pestalotiopsis are monophyletic. We report ITS length variations, single nucleotide polymorphisms (SNPs) and insertions/deletions (INDELS) among ten species of Pestalotiopsis that did not cause any phylogenetic error at either genus or species designation levels.? New gene sequences have been assigned (Gen Accession numbers from HM 190146 to HM 190155) by the National Centre for Biotechnology Information, USA.

Key words

Pestalotiopsis, Morphology, Host association, Molecular phylogeny, rDNA sequencing

Copyright ? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).