Journal of Environmental Biology

Studies on genetic divergence among Indian varieties

of a spice herb, Coriandrum sativum

Author Details

S.K. Singh

(Corresponding author)

Central Arid Zone Research Institute, Jodhpur - 342 003, India

e-mail: sksingh1111@hotmail.com

R.K. Kakani

National Research Centre on Seed Spices, Tabiji, Ajmer - 305 206, India

R.S. Meena

National Research Centre on Seed Spices, Tabiji, Ajmer - 305 206, India

Anjly Pancholy

Central Arid Zone Research Institute, Jodhpur - 342 003, India

Rakesh Pathak

Central Arid Zone Research Institute, Jodhpur - 342 003, India

Aparna Raturi

Central Arid Zone Research Institute, Jodhpur - 342 003, India

Publication Data

Paper received:

14 January 2011

Revised received:

19 April 2011

Accepted:

25 May 2011

Abstract

Coriander (Coriandrum sativum L.) is an annual spice herb that belongs to umbel family Apiaceae with diversified uses. We investigated the extent of variability among 22 Indian varieties of coriander using phenotypic and genetic markers. Multilocus genotyping by nine RAPD primers detected an average of intraspecific variations amounting to 66.18 % polymorphism in banding patterns. Analysis of molecular variance indicated that a greater proportion of total genetic variation exists within population (98%) rather than among populations (2%). Higher values of Nei?s gene diversity (h) and Shannon Information Index (i) and genetic distance analysis validate wider genetic diversity among Indian coriander varieties. Besides total internal transcribed spacer (ITS) length variations and single nucleotide polymorphisms, insertions/deletions (INDELS) were detected at seven sites in ITS-1 region. Multiple sequence alignment of 12 sequenced varieties revealed cent per cent identities of 5.8S gene region (162 bp) that validates its conserved nature. Multiple sequence alignment of ITS-1 region may be of phylogenetic significance in distinguishing and cataloguing of coriander germplasm. The representative sequences of each subgroup and all distinct varieties of RAPD clusters have been submitted to NCBI database and assigned Gen Accession numbers HQ 377194-377205. The measures of relative genetic distances among the varieties of coriander did not completely correlate the geographical places of their development. Eventually, the knowledge of their genetic relationships and DNA bar coding will be of significance. ?

Key words

Coriandrum sativum, Genetic diversity, rDNA diversity, DNA bar coding?

Copyright ? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).