Journal of Environmental Biology

In-vitro shoot regeneration via caulogenesis in fern, Pteris vittata L.

Author Details

Shastri P. Shukla

(Corresponding author)

Department of Plant Sciences, University of Hyderabad, Hyderabad - 500 046, India

e-mail: sp_shukla2002@yahoo.co.in

P. B. Khare

Pteridology Laboratory, National Botanical Research Institute, Lucknow - 226 001, India

Publication Data

Paper received:

26 July 2010

Revised received:

25 January 2011

Accepted:

16 February 2011

Abstract

An attempt was made to standardize the protocol for the shoot regeneration via caulogenesis in Pteris vittata L. employing leaf primordium explants. Calli were induced on Murashige and Skoog?s (MS) and Parkers and Thompson?s (P and T) media supplemented with different combinations of 2, 4-dichlorophenoxyaceticacid (2, 4-D), 6-benzylaminopurine (BAP), a-naphthalene acetic acid (NAA) and Indole - 3-acetic acid (IAA). A combination of full strength MS medium with 2, 4-D (2.26 ?M) and BAP (2.22 ?M) was found to be ideal for profuse callusing (80%) against other combinations. More shoot differentiation (2.8?0.06) was achieved from the calli on one-fourth strength of P and T media fortified with BAP (4.44 ?M) and? NAA (2.68 ?M) when compared to other combinations but statistically not significant. ?

Key words

Pteris vittata, Shoot regeneration, Arsenic hyperaccumulator, Caulogenesis?

Copyright ? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).