Journal of Environmental Biology

Colonization of probiotic bacteria and its impact on

ornamental fish Puntius conchonius

Author Details

K.R. Divya

(Corresponding author)

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679 586, India

e-mail : divyaravi27@gmail.com

A. Isamma

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679 586, India

V. Ramasubramanian

Unit of Aquatic Biotechnology and Livefeed Culture, Department of Zoology, Bharathiar University, Coimbatore - 641 046, India

S. Sureshkumar

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679 586, India

T.S. Arunjith

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679586, India

Publication Data

Paper received:

23 July 2010

Revised received:

07 April 2011

Re-revised received:

06 June 2011

Accepted:

14 June 2011

Abstract

The present study was conducted to assess the establishment and effect of probiotic bacteria such as Bacillus coagulans, Bacillus mesentericus, and Bifidobacterium infantis in the gut of freshwater ornamental fish Puntius conchonius. Postlarvae of 60 days old Puntius conchonius divided in four experimental groups each with three replicates. T₁, T₂ and T₃ groups were fed with Bacillus coagulans, Bacillus mesentericus and Bifidobacterium infantis enriched copepod Thermocyclops decipiens respectively. T₀ was the control group (without probiotic treated T.decipiens). The experiment was conducted for 45 days. The initial gut analysis of fish showed significant level of pathogenic bacteria in the gut of fish (p<0.05).? Total plate count of initial gut analysis of fish larvae were enumerated as 1.2 x 10⁴ CFU ml^-1.? The bacteriological study indicated that final gut microflora of post-larvae have decreased level of pathogens. Total plate count of T₁, T₂, T₃ and T₀ were reported to be as 1.08x10⁴, 1.06x10⁴, 1.27x10⁴ and 2.1x10⁵ CFU ml^-1, respectively. Three experimental groups (T₁, T₂ and T₃) were significantly different from control group (T₀) (p<0.05). At the end of the experiment, establishment of probiotics were examined. One week after probiotics administration, gut analysis of fish larvae showed, poor spore formation of Bacillus coagulans (2.3x10? CFU ml^-1), when compared to B. mesentericus (3.2?0.03x10? CFU ml^-1) and Bifidobacterium infantis (3.1x10^? CFU ml^-1). The results from the study suggest that the probiotic bacteria significantly established in gut of P. conchonius and significant effects on the pathogenic gut inhabitants of the fish. ??

Key words

Probiotics, Enrichment, Puntius conchonius, Thermocyclops decipiens?

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