Journal of Environmental Biology

Hydrogen peroxide-scavenging enzymes impart tolerance to high

temperature induced oxidative stress in sugarcane

Author Details

Sangeeta Srivastava

(Corresponding author)

Division of Crop Improvement, Indian Institute of Sugarcane Research, Lucknow - 226 002, India

e-mail: sangeeta_iisr@yahoo.co.in

Ashwini Dutt Pathak

Division of Crop Improvement, Indian Institute of Sugarcane Research, Lucknow - 226 002, India

Prashant Shekhar Gupta

Division of Crop Improvement, Indian Institute of Sugarcane Research, Lucknow - 226 002, India

Ashok Kumar Shrivastava

Division of Plant Physiology and Biochemistry, Indian Institute of Sugarcane Research, Lucknow ?

226 002, India

Arun Kumar Srivastava

Agrometeorology Unit, Indian Institute of Sugarcane Research, Lucknow - 226 002, India

Publication Data

Paper received:

14 December 2010

Revised received:

30 May 2011

Accepted:

14 June 2011

Abstract

Seventy-one genotypes of sugarcane from diverse agro-climatic zones of India viz. peninsular, northwest, north-central and eastern zones, were screened for their tolerance to high temperature stress based on the damage to leaf biomass i.e. necrosis of leaf-tips and margins, and rolling of leaves. Nine selected genotypes showing variable response to heat injury were tested for activity pattern of isoforms of two H₂O₂-scavenging enzymes; ascorbate peroxidase (APX) and catalase (CAT), under high temperature induced oxidative stress. Changes in the activity of APX and CAT isozymes in leaves corresponded to the level of tolerance of genotypes towards heat injury which was substantiated by the highly negative correlation coefficients of heat injury levels of leaves vs. integrated density of APX and CAT isozyme bands. This indicated that the criteria of higher expression of CATs? and APXs?, the two major reactive oxygen species scavenging proteins in leaves may be used to screen large seedling populations and germplasm for high temperature tolerance. ??????

Key words

Saccharum spp. (hybrids), H₂O₂-scavengers, Heat tolerance, Oxidative stress and Reactive oxygen species ??

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