Journal of Environmental Biology

Reproductive activities of Heliotropium indicum isolate against

Helopeltis theivora and toxicity evaluation in mice

Author Details

A.K. Dolui

Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh ? 786004, India.

Manabendra Debnath

(Corresponding author)

Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh ? 786004, India. ???

e-mail : manab_d@rediffmail.com

B. De

Regional Institute of Pharmaceutical Science and Technology, Agartala ? 799005, India.

Atul Kumar

Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh ? 786004, India.

Publication Data

Paper received:

07 October 2010

Revised received:

19? July 2011

Accepted:

06? August 2011

Abstract

A new compound E was isolated from the methanolic extract of the leaves of Heliotropium indicum by chromatographic fractionation. In the present study, the effect of the compound E on reproduction of Helopeltis theivora has been evaluated. The acute toxicity study (LD₅₀) and sub-acute toxicity studies (haematological, biochemical and histopathological parameters) in albino Swiss mice were carried out to evaluate the safety aspect of the compound E. The compound showed significant inhibitory effect on the reproductive life of H. theivora. The oviposition period, fecundity and hatching percentage of H. theivora were found to be 15.67days, 39.33 and 28.00% respectively after treatment with 2% compound E, whereas the control value were found to be 20.33 days, 77.67 and 77.33% respectively. The LD₅₀ of the compound was found to be 780 mg kg^-1 in Swiss albino female mice. The compound did not show any toxicity in mice at sub-lethal dose treatment (78 mg kg^-1 b. wt., once daily) for 21 days as evident from different haematological, biochemical and histopathological parameters in compound E treated group when compared with control.? ??????

Key words

Helopeltis theivora, Insecticidal properties, Heliotropium indicum, Reproductive inhibition. ???

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