Journal of Environmental Biology

Transferability of STS markers in studying genetic relationships

of marvel grass (Dichanthium annulatum)

Author Details

Raghvendra Saxena

Crop Improvement Division, Indian Grassland and Fodder Research Institute,

Jhansi - 284 003, India

Amaresh Chandra

(Corresponding author)

Crop Improvement Division, Indian Grassland and Fodder Research Institute,

Jhansi - 284 003, India

e-mail: amaresh62@yahoo.com

Publication Data

Paper received:

04 November 2009

Revised received:

31 March 2010

Accepted:

19 April 2010

Abstract

Transferability of sequence-tagged-sites (STS) markers was assessed for genetic relationships study among accessions of marvel grass (Dichanthium annulatum Forsk.). In total, 17 STS primers of Stylosanthes origin were tested for their reactivity with thirty accessions of Dichanthium annulatum. Of these, 14 (82.4%) reacted and a total 106 (84 polymorphic) bands were scored. The number of bands generated by individual primer pairs ranged from 4 to 11 with an average of 7.57 bands, whereas polymorphic bands ranged from 4 to 9 with an average of 6.0 bands accounts to an average polymorphism of 80.1%. Polymorphic information content (PIC) ranged from 0.222 to 0.499 and marker index (MI) from 1.33 to 4.49. Utilizing Dice coefficient of genetic similarity dendrogram was generated through un-weighted pair group method with arithmetic mean (UPGMA) algorithm. Further, clustering through sequential agglomerative hierarchical and nested (SAHN) method resulted three main clusters constituted all accessions except IGBANG-D-2. Though there was intermixing of few accessions of one agro-climatic region to another, largely groupings of accessions were with their regions of collections. Bootstrap analysis at 1000 scale also showed large number of nodes (11 to 17) having strong clustering (>50). Thus, results demonstrate the utility of STS markers of Stylosanthes in studying the genetic relationships among accessions of Dichanthium.

Key words

Cluster analysis, Genetic resources, Marvel grass, Polymorphism, STS markers

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