Journal of Environmental Biology

Studies on influence of natural biowastes on cellulase

production by Aspergillus niger

Author Details

M. Usha Kiranmayi

(Corresponding author)

Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar,

Guntur - 522 510,? India

e-mail: ugali1974@gmail.com

Sudhakar Poda

Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar,

Guntur - 522 510, India

P.B.B.N. Charyulu

Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515 001, India

M. Vijayalakshmi

Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar,

Guntur - 522 510, India

P.V. Krishna

Department of Zoology and Aquaculture, Acharya Nagarjuna University, Nagarjuna nagar,

Guntur - 522 510, India

Publication Data

Paper received:

11 March 2010

Revised received:

22 November 2010

Accepted:

20 December 2010

Abstract

The objective of this study was to determine the influence of natural biowaste substrates such as? banana peel powder and coir powder at varying environmental parameters of pH (4-9) and temperature (20-50^oC) on the cellulase enzyme production by Aspergillus niger. The cellulase enzyme production was analyzed by measuring the amount of glucose liberated in IU ml^-1 by using the dinitrosalicylic acid assay method. The substrates were pretreated with 1% NaOH (alkaline treatment) and autoclaved.? The maximum activity of the enzyme was assayed at varying pH with temperatures being constant and varying temperatures with pH being constant. The highest activity of the enzyme at varying pH was recorded at pH 6 for banana peel powder (0.068 + 0.002 IU ml^-1) and coir powder (0.049 ? 0.002 IU ml^-1) and the maximum activity of the enzyme at varying temperature was recorded at 35^oC for both banana peel powder (0.072 ?0.001 IU ml^-1) and coir powder (0.046 ?0.003 IU ml^-1). At varying temperatures and pH the high level of enzyme production was obtained at 35^oC and pH 6 by using both the substrates, respectively. However among the two substrates used for the production of cellulases by Aspergillus niger banana peel powder showed maximum enzymatic activity than coir powder as substrate.

Key words

Natural biowaste, Cellulase activity, Aspergillus niger

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