Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Nov 2011, 32 (6) Back
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Characterization of protease
from Alcaligens faecalis and its antibacterial
activity on fish pathogens Author Details |
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(Corresponding
author) |
Faculty of Marine Science, CAS in Marine Biology, India e-mail:
annabact@gmail.com |
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Faculty
of Marine Science, CAS in Marine Biology, |
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Faculty
of Marine Science, CAS in Marine Biology, |
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Faculty
of Marine Science, CAS in Marine Biology, |
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Faculty of Marine Science, CAS
in Marine Biology, India |
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Publication Data Paper received: 06
August 2010 Revised
received: 05
December 2010 Accepted: 18 December 2010 |
Abstract Alcaligens faecalis AU01 isolated from seafood industry effluent produced an
alkaline protease. The optimum culture conditions for growth as well as
enzyme production were 37oC and pH 8. The partially purified
protease had specific activity of 9.66 with 17.77% recovery with the
molecular weight of 33 kDa and it was active
between 30-70oC and optimum being at 55oC and pH 9. The
enzyme retains more than 85% activity at 70oC and 78% even at pH
10.The enzyme inhibited the growth of fish pathogens such as Flavobacterium sp., Pseudomonas fluorescens, Vibrio
harveyi, Proteus sp. and Vibrio parahaemolyticus.
From the present study it can be concluded that Alcaligens
faecalis AU01 has the potential for
aquaculture as probiotic agent and other several
applications. Key
words Alkaline protease, Alcaligens faecalis, Antibacterial,
Seafood effluent, Fish pathogens? ? |
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