Journal of Environmental Biology

Changes in protein subunits induced by endosulfan and fenvalerate

in fresh water fish Labeo rohita through SDS-PAGE

 

K. Suneetha*¹, K.G. Kumar¹ and K. Veeraiah²

¹Department of Biochemistry, Acharya Nagarjuna University, Nagarjuna Nagar - 522 510, India

 

²Department of Zoology, Acharya Nagarjuna University, Nagarjuna Nagar - 522 510, India

(Received: March 13, 2009; Revised received: November 12, 2009; Re-Revised received: January 21, 2010; Accepted: February 23, 2010)

 

Abstract: Fresh water fish Labeo rohita was exposed to two pesticides i.e., endosulfan an organchlorine and fenvalerate a synthetic pyrethroid. The 1/10^th of 24 hr LC₅₀ of endosulfan (0.0687 m g l^-1) and fenvalerate (0.0 474 m g l^-1) were selected as sub lethal concentrations. The fish were exposed to sublethal concentrations for one week and the changes in the tissue proteins of vital organs such as brain, liver, gill? and muscle were studied under SDS-PAGE. The protein subunits were identified by running marker proteins parallel and R_m values were calculated accordingly. The changes in the protein banding pattern are more pronounced in the fenvalerate exposure than endosulfan.

Key words: SDS-PAGE, Electrophoretogram, R_m values

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