Journal of Environmental Biology

Assessment of genetic diversity of Clarias batrachus using

RAPD markers in three water bodies of Bhopal

 

R.K. Garg, P. Sairkar, N. Silawat N. Batav and N.N. Mehrotra*

 

Centre of Excellence in Biotechnology, M.P. Council of Science and Technology (MPCST)

Vigyan Bhawan, Nehru Nagar, Bhopal - 462 003, India

*Central Drug Research Institute, Lucknow - 226 001, India

(Received: August 06, 2009; Revised received: February 18, 2010; Accepted: February 24, 2010)

 

Abstract: To assess the extent of genetic diversity within and between three populations of the catfish, Clarias batrachus, an analysis for random amplified polymorphic DNA (RAPD) variations were carried out. Five out of ten random primers produced constant and reproducible pattern of RAPD products. These five primers produced 72 scorable DNA fragments out of which 68 (86.66%) were polymorphic. Populations showed significant differences in their degree of polymorphism. Differences however, were observed in the primers in producing similarities in the populations.

Key words: Catfish, Genetic diversity, Similarity coefficient, RAPD, Polymorphism

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