Journal of Environmental Biology

Effective method for extraction of larvicidal component from leaves of Azadirachta indica and Artemisia annua Linn.

 

Shobhita Tonk¹, Roli Bartarya¹, K. Maharaj Kumari¹, V. P. Bhatnagar² and S. S. Srivastava¹

¹Department of Chemistry, ²Department of Botany,

Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-282 005, India

 

(Received: 27 August, 2004 ; Accepted: 9 February, 2005)

 

Abstract: Leaves of Artemisia annua linn. and Azadirachta indica were extracted in petroleum ether and hexane respectively by different methods of extraction i.e. cold extraction, reflux extraction and soxhlet extraction. The crude extract obtained was tested against third instar larvae of Anopheles stephensi. On comparison of larval mortality of crude extract obtained by these three methods, both? soxhlet and reflux extraction method showed 100 % mortality at 200 ppm after 48 hr in case of A. annua. However LC₅₀ (20 ppm) value of crude extract obtained by soxhlet extraction showed better results than reflux extraction (35 ppm) method after 72 hr. In case of A. indica, crude obtained by soxhlet showed 100% mortality (after 48 hr) at 250 ppm and LC₅₀ of 69 ppm at 72 hr. Reflux extraction does not show any appreciable mortality even at 250 ppm concentration.

 

Key words: Anopheles stephensi, Larvicidal activity, Crude extract, Reflux, Hot extraction, Cold extraction.

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