Journal of Environmental Biology

Stress behavior and biochemical profiling of Opsarius bendelisis under different photoperiods

J. Chand¹, N.S. Karki², J. Bhatt¹, S. Bohra¹ and B.C. Pathak¹*     

¹Department of Zoology, Motiram Baburam Govt. P. G. College, Haldwani-263 139, India

²Department of Chemistry, Laxman Singh Mahar Campus Govt. P. G. College, Pithoragarh-262 501, India

Received: 29 January 2024                   Revised: 09 April 2024                   Accepted: 08 May 2024

*Corresponding Author Email : bipinpathak11@gmail.com                  *ORCiD: https://orcid.org/000-0002-7987-5584

Abstract

Aim: The investigation evaluated the impact of varying photoperiods on the physiological regulation of stress pathway and the biochemical concentration of metabolites in hillstream trout, Opsarius bendelisis.

Methodology: Different light phases were used in the present study to quantify their weight and chronic stress marker cortisol using enzyme-linked immunosorbent assay (ELISA). The behavior of the fish was investigated using the highly sophisticated software Ethovision XT 13 with a frame rate of 25 per sec. The fish’s lipid profiles were measured using gas chromatography-mass spectrometry (GC-MS).

Results: The chronic marker of stress cortisol showed a relevant difference among the various regimes of photoperiod at P≤0.0046.The different observed behavior profiles, such as velocity distance traveled, also showed a significant difference at P<0.0001 in the different light-exposed durations. The 9-octadecenoic acid, methyl ester, (E)- (I), and hexadecanoic acid, methyl ester also showed a significant difference at P<0.05.

Interpretation: Extended photoperiod lowers stress levels and influences fish behavior. The results of this investigation will offer fresh perspectives on cognitive-behavioral tests.

Key words: Cortisol, ELISA, Opsarius bendelisis, Photoperiods, Stress behavior

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