study of Himalayan plant extracts against oomycetes disease Saprolegniasis in
rainbow trout (Oncorhynchus mykiss)
N.K. Chadha1, P. Dash1,2, P.B. Sawant1, N.N.
Pandey2, S. Chandra2, R.A.H. Bhat1,2 and D.
Institute of Fisheries Education, Mumbai-400 036, India
of Coldwater Fisheries Research, Nainital-263 136, India
*Corresponding Author Email : email@example.com
This study aimed to investigate the effectiveness of ethanolic extract of
three Himalayan plants Myrica esculenta, Thymus linearis and Butea
monosperma on hyphal germination, colonisation and sporulation of two
species of Saprolegnia (Saprolegnia parasitica and S.
australis) isolated from rainbow trout, Oncorhynchus mykiss.
Molecular docking of active ingredients of M. esculenta, Myricetin
with effector proteins of S. parasitica was also performed to
investigate the target binding sites for drug development.
Methodology: Minimum inhibitory concentration (MIC), mycelium
growth inhibition, spore germination, and inhibition was performed with the
most effective concentrations. Molecular docking was carried out with
AutoDock Vina software to investigate target binding sites with S.
Extracts from Myrica esculenta, Thymus linearis and Butea
monosperma showed MIC values of the 25, 100, 50 mg ml-1
against S. parasitica and 25, 50, 25 mg ml-1 against S.
australis hyphal growth, respectively. Nevertheless, malachite green as
reference control was effective with a MIC value of 2.5 mg l-1.
The concentration required to inhibit S. parasitica and S.
australis spores were (50) Myrica esculenta, (25) Thymus
linearis, (100) Butea monosperma in mg ml-1 and (50) Myrica
esculenta, (50) Thymus linearis, (100) Butea monosperma in
mg ml-1, respectively.
Interpretation: The study concludes that M. esculenta
and B. monosperma are effective against Saprolegniasis and could be
used as phyto additives.
Key words: Anti-oomycetes, Plant extract, Saprolegnia
australis, Saprolegnia parasitica, Spore germination
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