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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Mar 2021, 42 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Development of species-specific marker and molecular diversity of Conogethes punctiferalis and Conogethes sahyadriensis (Lepidoptera: Crambidae)

                                                                                                                 

V. Kammar1, D. Sagar2*, R.K. Chandel2, P.R. Shashank2 and A.K. Chakravarthy3

1Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore- 560 065, India

2Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India

3Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Bangalore-560 089, India

*Corresponding Author Email : garuda344@gmail.com

 

Received: 06.11.2019                                                                   Revised: 29.07.2020                                                Accepted: 24.10.2020

 

 

Abstract

Aim: The aim of this study was to develop species specific marker for quick identification of two Conogethes species and to study their molecular diversity.

Methodology: Species-specific markers were developed using mitochondrial cytochrome oxidase 1 (COI) partial gene sequences of Conogethes punctiferalis and C. sahyadriensis and they were validated. Phylogenetic tree was constructed using MEGA X program, tree robustness was evaluated by bootstrapping with 1000 replicates with all sequences of C. punctiferalis and C. sahyadriensis.   

Results: Bioinformatic analysis of C. punctiferalis and C. sahyadriensis COI sequences revealed that they have sufficient genetic variability to develop species specific marker. The COI based species-specific markers amplified an expected fragment size of 333 bp for C. punctiferalis and 522 bp for C. sahyadriensis, which clearly differentiate these two species.                         

Interpretation: The species specific marker developed in this study will be useful in quick identification of C. punctiferalis and C. sahyadriensis. The use of COI gene as a marker can provide a better estimate of genetic diversity, as it is maternally inherited and can be helpful to understand evolutionary processes.        

Key words: Conogethes punctiferalis, C. sahyadriensis, MtCOI, Molecular diversity, Species-specific marker

 

 

 

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