Abstract
Aim:
The experiment was aimed to study the effects of various Zn formulations
towards induced host plant resistance to WBPH in rice.
Methodology: Eight zinc treatments comprising basal and foliar
applications(at 30 and 45 DAT) of Zn SO4 and Zn-EDTA and their
combinations were tested for induced host plant resistance to WBPH (a
dreadful sucking insect) in a most sensitive rice variety TN 1.
In addition, a treatment without any Zn application served as control. The
pot grown plants were kept in the Mylar cages with top end covered by fine
mesh muslin cloth and as such maintained till maturity. One adult gravid
female insect was released into each cage (at 46DAT) and the insect
population build-up was recorded after one month. Soluble protein profiling
of leaf samples (at 60DAT) of each treatment was done by SDS-PAGE and data
were analyzed for clustering pattern.
Results:
Altogether, 19 polypeptide bands (14.3-97.4kDa) were revealed. The low
molecular weight proteins (14.3-25.1 kDa) were clearly absent in the
control. T6(Zn EDTA at basal and foliar) recorded least WBPH
build–up and elicited highest number (15) of polypeptide bands including five
new bands at 66.0, 37.0, 23.6, 15.8 and 14.3 kDa. Further, 66.0kDa, 37.0 kDa
and 14.3 kDa polypeptide bands were commonly shared by T6, T7(ZnSO4
basal+ EDTA foliar) and T8(Zn EDTA basal + ZnSO4) that
recorded lower WBPH population and grouped together as compared to rest of
the treatments and control. However, 23.6 kDa polypeptide band induced only
in T6 and T7 seems to have greater role in
manifestation of induced defence mechanism against WBPH in
rice.
Interpretation: The differentially expressed proteins
(as compared to control) revealed in response to Zn application may be considered
as biochemical basis of induced resistance for the pest in rice
Key
words:
Induced resistance, Soluble protein profiling, White backed plant hopper,
Zinc
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