Journal of Environmental Biology

New cost-effective mediator enhanced enzymatic degradation of aniline blue

C. Lü¹, X.F. Luo², X.J. Dong¹, J.Q. Peng¹ and F.X. Cao¹*

¹School of Life Science and Technology, Central South University of Forestry and Technology, Changsha-410 004, China

²School of Chemical and Biological Engineering, Hunan University of Science and Engineering, Yongzhou-425 000, China

*Corresponding Author Email : klhlv@126.com

Received: 05.03.2020                                                                     Revised: 08.07.2020                                                          Accepted: 26.08.2020

Abstract

Aim: In this study, the effects of various agricultural wastes as low-cost, readily available alternatives to chemical mediators for the enzymatic degradation of aniline blue were investigated.

Methodology: Enzymes for degrading aniline blue were obtained through solid-state fermentation (SSF) of Pleurotus eryngii. Agricultural waste including grape skin/seed and orange peel, rich in organic acids, were used instead of chemical reagents as mediators. The degradation products were subjected to liquid chromatography-mass spectrometry (LC-MS) analysis.

Results: The results show that the enzymes manganese peroxidase and versatile peroxidase were associated with aniline blue degradation. The organic acid-rich agricultural waste (grape seed/skin, orange peels) serving as substitutes for chemical reagents as mediators reduced the cost of the mediator by 99.8% at best, and the 2 hr degradation rate of aniline blue was 97.4% and 95.8%, respectively, increased by at least 4.3%. The LC-MS analysis revealed that aniline blue was degraded into smaller compounds with significantly lower toxicity.          

Interpretation: Overall, the enzyme–organic acid-rich agricultural waste mediator (grape seed/skin) system effectively degraded aniline blue. This cheap and efficient method for aniline blue degradation can serve as an environmentally friendly technology to treat waste with waste.         

Key words: Agricultural waste, Aniline blue, Enzymatic degradation, Organic acids, Pleurotus eryngii

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