Journal of Environmental Biology

Antimicrobial and anti-tubercular potential of Streptomyces lomondensis SACC 63 isolated from soil samples in Himachal Pradesh, India

G. Soundarya¹, K. Manigundan², P.R. Meganathan³, S. Gomathi⁴, S. Balaji⁴, J. Jerrine² and M. Radhakrishnan²* 

 ¹Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram-608 002, India

²Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai-600 119, India

³CSIR-National Environmental Engineering Research Institute, Hyderabad Zonal Centre, Hyderabad-500 007, India

⁴Department of Bacteriology, National Institute for Research in Tuberculosis, Chennai-600 031, India

*Corresponding Author Email : mrkactinos@gmail.com

Paper received: 27.12.2018??????? ?????????????????????????????????????? Revised received: 30.04.2019????????????? ?????????????????????????????? Accepted: 09.09.2019

Abstract

Aim: The antimicrobial and anti-tubercular potential of Actinobacteria isolated from soil in Himachal Pradesh, India, was examined in this study.

Methodology: The bioactive metabolites produced from five selected Actinobacterial cultures SACC-63, SACC-75, SACC-76, SACC-77 and SACC-N10 by agar surface fermentation were tested for their antimicrobial and anti-tubercular efficacy in in-vitro. The taxonomy of SACC-63 was identified as Streptomyces lomondensis using 16S-rRNA sequence analyses.???     

Results: The ethyl acetate extract of strain SACC-63 showed broad spectrum activity against wide range of bacterial and fungal pathogens at 250 ?g per disc concentration. In anti-tubercular screening by luciferase reporter phage (LRP) assay, four actinobacterial strains showed more than 65% inhibition against the standard strain Mycobacterium tuberculosis H37Rv and multi drug resistant (MDR) M. tuberculosis strain at 100 ?g ml^-1 and 500 ?g ml^-1 concentrations, except the strain SACC-N10. The MIC values of SACC-63 against microbial pathogens ranged between 62.5 ?g ml^-1 and 250 ?g ml^-1. The strain SACC-63 showed 99% similarity with Streptomyces spp. Further, phylogenetic analysis based on 16S rRNA revealed a close relationship between SACC-63 and S. lomondensis.     

Interpretation: The present study suggested that S. lomondensis SACC-63 isolated from Himachal Pradesh region in India could be a promising source for the production of bioactive molecules.       

Key words: Antimicrobial, Anti-tuberculosis, Bioprospecting, Luciferase reporter phage assay, Streptomyces species

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