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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Sep 2019, 40 (5)                                     Back


nstantaneous and historical temperature effects on a-pinene

Effect of cypermethrin on reproductive efficacy in zebrafish

(Danio rerio): In-vivo and in-silico studies

 

Paper received: 19.11.2018??????? ??????????????????????????????????????????????????? Revised received: 26.03.2019???????????? ????????????????????????????????????????????? Accepted: 30.04.2019

 

 

Authors Info

G.K. Pitchika1,2, K.V. Swamy3,

T.S. Ranjani1, C.R. Tyler4,

S.B. Sainath5 and G.H. Phillip1*     

  

1Department of Zoology, Sri

Krishnadevaraya University,

Anantapur-515 003, India

2Department of Zoology, Vikrama

Simhapuri University PG Centre,

Kavali-524 201, India

3Department of Bioinformatics

and Computer Science,

Dr. D.Y. Patil Biotechnology

and Bioinformatics Institute,

Pune-411 033, India

4Biosciences, College of Life and

Environmental Sciences, University

of Exeter, Exeter, EX4 4QD, UK

5Department of Biotechnology,

Vikrama Simhapuri University,

Nellore-524 320, India

 

    

*Corresponding Author Email :

pitchika.gopikrishna@gmail.com

 

 

 

Abstract

 

Aim: The aim of the present study was to evaluate the reproductive efficacy of male and female zebrafish following cypermethrin exposure.    

 

Methodology: The adult zebrafish (Danio rerio) of both sexes were exposed to cypermethrin at three selected concentrations 0.1, 1.0 and 10 ?gl-1 over a period of 21days. After completion of experimental period, the reproductive endpoints such as fecundity, hatchability, testis and ovarian histology and plasma vitellogenin levels were selected and determined in this study.    

 

Results: Cypermethrin exposure did not affect the cumulative fecundity rates in experimental fishes over controls. However, cypermethrin at 10 ?gl-1 showed a significant reduction in the sperm number in male fishes over control. On the other hand, the same concentration of cypermethrin did not show significant changes in the plasma vitellogenin levels of both male and female fishes over their respective controls. Analysis of testicular and ovarian architectures of male and female zebrafish exposed to cypermethrin at 10 ?gl-1 showed no marked differences over controls. In addition, molecular docking studies revealed that the binding energy between the cypermethrin and zebrafish estrogen receptor (zfER) β1 was almost similar to the binding energies exhibited by reference molecules, estradiol and ethinyl estradiol with zfERβ1. Further, binding energies between the ligands (cypermethrin and its metabolites phenoxybenzaldehyde and 3-phenoxybenzoic acid) with zfERα were low as compared to the binding energies between the reference molecules and zfERα.    

 

Interpretation: In-vivo studies indicated that cypermethrin at 10?g l-1 leads to spermatotoxicity in zebrafish and in silico analysis showed that the cypermethrin at least in part interfere with the signalling of zfERα.

 

Key words: Cypermethrin, Estrogen receptors, Spermatogenesis, Zebrafish

 

 

 

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