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Authors
Info
E. R. Sathendra1, G.
Baskar2* and R. Praveenkumar1
1Department of
Biotechnology, Arunai Engineering College, Tiruvannamalai-606 603, India
2Department of
Biotechnology,
St. Joseph's College of Engineering,
Chennai-600 119, India
*Corresponding
Author Email :
basg2004@gmail.com
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Abstract
Aim: Bioethanol
production from lignocellulosic material (banana peduncle) as a substrate
using microorganism Klyveromyces marxianus MTCC 1389 was carried out
in this study.
Methodology: The substrate was
prepared by mechanical grinding, followed by dilute acidic treatment using H2SO4
with various concentrations such as 1, 2 and 3%, respectively. Temperature
was maintained at 150?C for breaking the glycosidic linkage and
converting into monomer. The chemical composition and concentration of
various constituents in the raw material was characterized. Fermentation was
carried out at various temperatures (25, 30, 35 and 40?C) and pH
(4.0, 4.5, 5.0 and 5.5). Batch fermentation kinetics of bioethanol production
was studied.
Results: The maximum
biomass and ethanol yield was achieved at 40?C and pH 4.5 with
specific growth rate (μ) 0.021 hr-1, biomass yield (Yx/s)
14.7 g l-1 and bioethanol yield (Yp/s) 21.89 g l-1.
Interpretation: Lignocellulosic
material obtained from waste banana peduncle can be utilized for bioethanol
production.
Key words: Acid hydrolysis, Banana peduncle, Bioethanol, Klyveromyces
marxianus, Lignocellulose
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