G. Kumar and M. Bhardwaj*
Laboratory, Department of Botany, University of Allahabad, Prayagraj-211 002,
Author Email :
Aim: The present study
was conducted to investigate the genotoxic effect of UV-B radiation on
morphological, biochemical and cytological parameters of Cuminum cyminum.
Methodology: The germinated
roots of cumin were exposed to UV-B radiation at different intervals viz.,
20 min 40 min and 60 min, respectively, and some treated seeds were sown on
pots for morphological and biochemical observation.
studies divulged that UV-B radiation has substantial impact on Active Mitotic
Index (AMI %). On increasing UV-B radiation dose, the chromosomal aberration
rate elevated, thus AMI % moderately decreased and Total Abnormality
percentage (TAB %) gradually increased. Different types of chromosomal
abnormalities were ascertained, among which scattering was more prominent.
The morphological observation showed that survival percentage and plant
height decreased at elevated dose of UV-B. Biochemical results indicated that
chlorophyll a, chlorophyll b and carotenoid contents decreased as compared to
control, but proline content showed significant increment at higher doses of
Interpretation: The observation
elucidates that UV-B causes chromosomal aberrations during cell division and
acts as a potent genotoxic agents for roots. Thus, it can be concluded from
the above experiment that UV-B rays promote plant growth at low doses but at
the higher doses, it subsequently hinders plant growth by damaging important
constituents of plant cell.
Key words: Active mitotic index, Chromosomal abnormality, Cuminum
cyminum, Total abnormality percentage, UV-B rays