Y.E. Jeong1 and M.Y. Lee1,2*
Medical Science, College of Medical Sciences, Soonchunhyang University, 22,
Soonchunhyang-ro, Asan, Chungnam-31538, Republic of Korea?
2Department of Medical
College of Medical
Sciences, Soonchunhyang University, 22, Soonchunhyang-ro, Asan,
Chungnam-31538, Republic of Korea
Author Email :
Aim: The aim of the
present study was to measure in-vitro DNA and cell damage induced by
5-chloro-2-methyl-4-isothiazolin-3-one (CMIT), one of the humidifier
disinfectants, and to investigate the suppressive effect of various
phytochemicals against CMIT toxicity.
comet assay was performed to determine the degree of CMIT-induced DNA damage
at single cell level by measuring the olive tail moment. Upon treating CMIT
on the rat lymphocytes, the inhibitory effects of Vitamin C and several
phytochemicals such as berberine, curcumin and resveratrol were assessed. In
addition, MTT assay was used to examine the protective effect of resveratrol
on CMIT-induced cytotoxicity in cultured lung cells.
Results: In in-vitro
comet assay, the increased olive tail moment induced by CMIT was effectively
inhibited by Vitamin C, berberine, curcumin and resveratrol treatment.
Especially, resveratrol showed the best suppressive effect against DNA damage
by CMIT. In MTT assay, resveratrol also showed significant suppressive effect
against cytotoxicity induced by CMIT in cultured lung cells.
such as Vitamin C, berberine, curcumin and resveratrol can be utilized in the
development of preventive or therapeutic compositions against the injury
caused by CMIT toxicity.
Key words: Chloro methyl isothiazolinone, Comet assay, DNA damage,
MTT assay, Phytochemicals