on microbial and fungal contamination of air and wooden surfaces inside of a
historical Church from Romania
Ilieș1, A. Oneț2*, J.A. Wendt3, M.
Ilieș4, A. Timar2, A. Ilieș1, Ș.
Baias1 and G.V. Herman1
Geography, Tourism and Sport, University of Oradea, Oradea, 410087, Romania
Environmental Protection, University of Oradea, Oradea, 410048, Romania
Oceanography and Geography, University of Gdańsk, Gdańsk, 80-309,
Geography, University ``Babes Bolyai`` Cluj Napoca, Extension Sighetu
Marmatiei, 435500, Romania
Author E-mail: firstname.lastname@example.org
Paper received : 25.05.2017
Revised received : 23.10.2017
Re-revised received :
Accepted : 06.02.2018
Aim: The degree of
microbial contamination of air and surfaces reflect a potential risk of
disease which increases proportionally with the density of microorganisms and
the presence of pathogenic or potentially pathogenic species. The paper focus
on the degree of bacterial and fungal contamination of air and surfaces
inside? a historical wooden church from Oradea city, Romania.?? ?
Methodology: The conventional
technique of open plates (Koch sedimentation method) was used for the
microaeroflora inside the wooden church. The air samples were taken from two
places (from the middle of the room and from a corner, both places being at
the same height), during different times of the day. In order to determine
the fungi types present on the wood surfaces, dust samples were collected from
five places inside the wooden church. ?
Results: The results
obtained showed that the bacterial contamination of air had reached the
maximum limit of 100 CFU/4 hrs, meanwhile fungal contamination had exceeded
the contamination level C which provides 50 CFU/4 hrs according to the
European Union Guide to Good Manufacturing Practice (1997). The fungi
identified in the air inside of the wooden church belong to the genus : Aspergillus,
Penicillium, Stachybotrys, Scopulariopsis, Arthrinium, Mucor, Rhizopus,
Absidia and Geotrichum. ??
Interpretation: The results
obtained from the analysis of microaeroflora inside the wooden church
highlights the presence of various species of fungi with potential health
risk at worship place. The environmental conditions present within the church
favours the development of fungi and bacteria.
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