Abrogation
of sodium arsenite driven uterine antioxidant exhaustion and tissue
impairment: Role of B12 and folate
B.
Deb1, M. Maity1, S. Maiti2, B. Pan1,
H. Perveen1, M. Dash1, A.K. Maiti3 and S.
Chattopadhyay1*
1Department of
Biomedical Laboratory Science and Management and Clinical Nutrition and
Dietetics Division, Vidyasagar University, Midnapore-721 102, India
2Department of
Biochemistry, Oriental Institute of Science and Technology, Midnapore-721
102, India
3Department of
Pathology, Midnapore Medical College and Hospital, Midnapore-721 102, India
*Corresponding
Author E-mail: sandipdoc@mail.vidyasagar.ac.in
|
Key
words
Folic acid
S-adenosine methionine pool
sodium arsenite
uterine oxidative stress
vitamin B12
Publication Data
Paper received : 16.06.2017
Revised received : 23.10.2017
Re-revised received :
28.11.2017
Accepted : 28.12.2017
|
Abstract
Aim: Rapid emergence
of arsenic pollution demands the development of novel adjunct with non-invasive
painless oral therapeutic agent to combat against arsenic associated health
hazards by replacing conventional painful chelating therapy. In the present
study, the modulatory effects of vitamin B12 and folic acid
(folate) in the amelioration of arsenic mediated uterine disorders were
examined.?
Methodology: In this
experimental study, Wistar strain adult female rats were fed sodium arsenite
(As3+) contaminated water (0.4 ppm) in conjunction with vitamin B12
(0.04 μg and 0.07 μg) plus folate (2.0 and 4.0 μg) alone and
or in combination respectively per 100 g b.wt. per day for seven estrous
cycles (28 days).
Results: Rats those
underwent arsenic exposure showed significant impairments in the status of
uterine antioxidant profile as evident from enzymatic assay and electrozymogram
study of superoxide dismutase, catalase and peroxidase with an abnormal
increase in conjugated diene and malondialdehyde levels. Mutagenic uterine
DNA-breakage and tissue damages were prominent following As3+consumption
by the rats. All impairments were totally or partially attenuated by the
co-treatment with these two B vitamins in arsenic exposed rats.
Interpretation: The mechanisms
might be coupled with the enhancement of antioxidant defense system, partly
through the elimination of arsenic with the involvement of S-adenosine
methionine pool (SAM) since, vitamin B12 and folic acid are two
major regulators of this pool.
|
Copyright
? 2018 Triveni Enterprises. All rights reserved. No part of the Journal can
be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the conclusions
enforced or derived, rest completely with the author(s).
|