Journal of Environmental Biology

A simple and efficient micropropagation protocol for New Guinea Impatiens (Impatiens hawkeri)

L. Samiei¹*, M. Davoudi Panhehkolayi², H. Mirshahi¹ , and Z. Karimian¹

¹Department of Ornamental? Plants, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, 91775-1653, Iran

²Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, 91775-1653, Iran

*Corresponding Author E-mail: samiei@um.ac.ir

Key words

Apical bud

Benzyl amino purine

Impatiens hawkeri

Nodal explant

Kinetin

Publication Data

Paper received : 10.07.2017

Revised received : 03.11.2017

Re-revised received : 02.12.2017

Accepted : 22.12.2017?? ???

Abstract

Aim: The study aimed to compare the potentials for in vitro regeneration of apical and nodal explants of New Guinea Impatiens in the presence of cytokinins and to develop a simple, fast, cost-effective and efficient protocol for micropropagation of this valuable plant species. ???

Methodology: Due to high rate of contamination of Impatiens explants in in vitro conditions, a preliminary experiment was performed on surface sterilization of the bud explants using various sterilization reagents. In addition, the effects of various concentrations of benzyl amino purine and kinetin on proliferation of apical and axillary bud explants were investigated. Following two subcultures in multiplication media, the plantlets with well-developed roots were directly transferred to plastic cups and later to the greenhouse for acclimatization. ??????

Results: The most effective sterilization procedure, which resulted in 75% healthy explants, was recognized when the bud explants were disinfected with 0.1% mercuric chloride ? 6 min ? followed by a culture on Murashig and Skoog medium, consisting of 300 mgl^-1 Cefotaxime. Also, the results revealed that axillary buds were more efficient in inducing higher adventitious shoot regeneration compared to apical buds, when they were exposed to 0.5 mg l^-1 BAP in MS medium. In addition, the in vitro roots were simultaneously induced during shoot proliferation and maximum generated root number was observed in the medium containing 1 mg l^-1 BAP). The results showed that about 95% of the regenerated plantlets survived, acclimatized successfully and continued their normal and vigorous growth in the greenhouse. ?????    

Interpretation: The findings of this study indicated that the use of axillary buds in the presence of low concentrations of BAP were effective in multiplication of New Guinea Impatiens. The simultaneous occurrence of rooting during proliferation was beneficial in significantly reducing the time and cost of micropropagation procedure. The results propose a simple, fast, efficient and economic protocol that can be easily commercialized for micropropagation of Impatiens hawkeri 'Cherry Red'.

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