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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2018, 39 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Bioefficacy of antimicrobial peptide biosynthesis-gene-linked antagonistic Lysinibacillus sphaericus strains for management of bacterial plant diseases in Andaman and Nicobar Islands, India


K. Sakthivel1*, K. Manigundan1, R.K. Gautam1, P.K. Singh1, Yogeshwari1, T. Subramani1, S.K. Sharma2 and R. Khande3

1Division of Field Crop Improvement and Protection, ICAR- Central Island Agricultural Research Institute, Andaman & Nicobar Islands-744 101, India

2National Agriculturally Important? Microbial Culture Collection, ICAR-National Bureau of Agriculturally? Important Microorganisms, Mau Nath Bhanjan - 275 001, India

3Department of Biotechnology, Barkatullah University, Bhopal-462 026, India

*Corresponding Author E-mail:




Key words

Antagonistic activity

AMP biosynthesis genes

Biological control

Lysinibacillus sphaericus

Rhizosphere soil 


Publication Data

Paper received : 23.11.2016

Revised received : 11.05.2017

Re-revised received : 22.08.2017

Accepted : 13.10.2017 ?????      



Aim: The present study aimed to investigate the bioefficacy of antimicrobial peptide-gene-linked antagonistic Lysinibacillus sphaericus strains isolated from Andaman and Nicobar Islands, India for controlling diseases in plants.? ????   


Methodology: Bacillus spp. isolated from different niches of Andaman & Nicobar Islands were characterized for antimicrobial and PGP- traits. The identity of potential isolates was confirmed by 16SrRNA gene sequence analysis. Biocontrol potential of selected isolates against chili bacterial wilt pathogen was tested in glasshouse conditions. The antimicrobial peptide biosynthesis genes responsible for antagonistic activities of Lysinibacillus sphaericus strains were analysed by PCR based methods.? ?       


Results: Out of fifty two isolates tested, two Lysinibacillus sphaericus strains (Ls_Agu and NS2) collected from Nicobar sea sand and chili crop rhizosphere of South Andaman Islands were found good in in-vitro antagonistic potential against important plant bacterial pathogens: Ralstonia solanacearum (solanaceous bacterial wilt) and Xanthomonas oryzae pv. oryzae (rice bacterial blight) using an agar diffusion assay. In glasshouse study, both the strains Ls_Agu and NS2 showed significant biocontrol efficacy (88.9% and 66.7%) against Ralstonia solanacearum, which causes bacterial wilt disease in chili. The antimicrobial potential of these strains were further ascertained by presence of three AMP biosynthesis genes (surfactin, bacilysin and fengycin) in both the strains through PCR amplification. ?????          


Interpretation: The overall results revealed the antimicrobial peptide-gene-linked strains Ls_Agu and NS2 of L. sphaericus have ability to control bacterial plant disease under Islands conditions and the same strains could be utilized for popularization on Islands for management of the bacterial diseases.



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