Journal of Environmental Biology

Association of leaf micro-morphological features with

sterility mosaic disease resistance in pigeonpea

P.G. Patil^{1 ,2}*, H.E. Shashidhar², M. Byregowda², G.A.M. Reena², T.H. Ashok², H.V.V. Swamy², H.K. Ramappa² and J. Babu²?

¹Division of Plant Biotechnology, Indian Institute of Pulses Research, Kanpur-208 024, India

²Department of Plant Biotechnology, AICRP on Pigeonpea, University of Agricultural Sciences, GKVK, Bengaluru -560 065, India

*Corresponding Author E-mail: patilbt@gmail.com

Key words

Discriminant analysis,

Micro-morphological traits,

Pigeonpea,

Sterility mosaic disease

Publication Data

Paper received : 18.02.2016

Revised received : 25.07.2016

Re-revised received : 23.11.2016

Accepted : 01.12.2016

Abstract

Aim: Sterility mosaic disease (SMD) is one of the major disease in pigeonpea, which causes heavy yield losses upto 95 % in India. The disease is caused by pigeonpea sterility mosaic virus (PPSMV) and transmitted by eriophyid mite (Aceria cajani). The occurrence of five different isolates of virus were reported, which are transmitted by single biotype of mite. Therefore, the present study was carried out to identify important leaf structural features of pigeonpea that impart resistance to mite vector, there by helping in broad based resistance breeding against SMD pathogens.

Methodology: Twenty three extreme pigeonpea genotypes showing resistance (9) and susceptible (14) reaction to SMD were selected from seventy genotypes, which were screened for Bengaluru isolate of SMD. Data were recorded on twelve important leaf micro-morphological traits including trichome number and length on the upper and lower surface of leaf in cultivars and wild belonging to resistance and susceptible groups. The promising leaf features which were significantly differing, correlating and with higher discriminant function were identified from the resistant and susceptible groups of cultivars, wild and across using three statistical methods such as paired t-test, correlation and discriminant analysis.?

Results: In cultivars, specific leaf area (SLA) and specific leaf weight (SLW), in wild trichome number on upper surface (TNUS) and lower surface (TNLS) of leaf, and for across genotypes trichome length on lower surface of leaf (TLLS) and SLA were found most informative. Association of these traits across the genotypes revealed that TNUS, TNLS, TLLS and SLW were significantly and negatively associated with percent disease incidence (PDI). Considering these traits, discriminant functions were developed to identify resistant genotypes. Scatter plots based on SLA and SLW classified pigeonpea cultivars with 100% accuracy, TNLS and TNUS classified wild genotypes with 83.3% accuracy, TLLS and SLA classified both cultivar and wild with 76.6 % accuracy in relation to disease response. 

Interpretation: The leaf micro-morphological features such as TNUS, TNLS, TLLS, SLA and SLW were found significantly differing, correlating and with higher discriminant function in relation to SMD disease response. These traits are possibly involved in bringing vector mediated resistance to SMD.?????? ??????????

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