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Abstract - Issue Nov 2016, 37 (6) Back
nstantaneous and historical temperature effects on a-pinene
diversity assessment of Fusarium oxysporum f. sp ciceris isolates of
Indian chickpea fields as revealed by the SRAP marker system
Soren1*, Priyanka Gangwar1, Payal Khatterwani1,
Ram Ganesh Chaudhary2 and Subhojit Datta3
1Division of Plant
Biotechnology, Indian Institute of Pulses Research, Kanpur-208 024, India.
2Division of Plant
Protection, Indian Institute of Pulses Research, Kanpur-208 024, India.
Institute for Jute and Allied Fibers, Barrackpore-700 120, India.
Author E-mail: email@example.com
25 August 2015
19 December 2015
12 February 2016
experiment was conducted to study the precise geographical distribution and
racial complexity of Fusarium oxysporum f.sp ciceris (Foc) isolates
representing 12 states of 4 agro-climatic zones of India at morphological,
pathogenic and molecular level. The DNA based sequence related amplified
polymorphism (SRAP) markers was employed to differentiate Foc isolates at
genome level. The genotypic data output of the isolates was examined for
diversity parameter as marker's Polymorphic percentage (PM %), Polymorphic
Information Content (PIC), Marker Index (MI) and Gene Diversity Index (DI).
As a result, 15 primers used in this study could generated total of 154
reproducible alleles ranging from 100-2100 bp (average allele per marker
10.26) in size, of that 149 (97%) were found to be polymorphic. The
neighbor-joining analysis effectively classified the isolates of North East
Plain Zone (NEPZ), Central Zone (CZ), North West Plain Zone (NWPZ) and South
Zone (SZ) into four clusters. In summary, DNA based marker analysis could
differentiate as per isolates geographical location, however pathogenic
interaction of isolates from same geographical location could not match the
genetic differentiation. Accordingly, considering the present complexity in
racial profile, precise classification based on homologs virulence genes specific
to races would give a more meaningful in correlating isolates with their
native geographical distribution and helps in future resistance breeding
programs for sustainable management of vascular wilt disease.
diversity, Focisolate, Pathogenicity, Population structure, Racial profiling,
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