Journal of Environmental Biology

Activities of cell wall peroxidases in relation to lignification in six wheat (Triticum aestivum L.) genotypes under heat and drought stress

S. Bala¹, B. Asthir¹* and N.S. Bains²

¹College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana-141 004, India

²College of Agriculture, Punjab Agricultural University, Ludhiana-141 004, India

*Corresponding Author E-mail: b.asthir@rediffmail.com

Publication Data

Paper received:

21 February 2015

Revised received:

21 July 2015?

Re-revised received:

30 October 2015

Accepted:

01 December 2015

Abstract

Sensitivity of grain developmental stages to high temperature and drought stress was investigated in six wheat genotypes (PBW 343, PBW 621, PBW 550, PBW 175, C 306 and HD 2967) in terms of peroxidases induced lignification. Drought and high temperature accentuated activities of coniferyl alcohol peroxidase [(CPX) (3.4-5.9 m mol min^-1 g^-1 f.wt.)] and syringaldazine peroxidase [(SPX) (0.13-0.59 m mol min^-1 g^-1 f.wt.)] and guaiacol peroxidase [(GPX) (0.11-0.59 m mol min^-1 g^-1 f.wt.)]. Histochemical localization of lignin in certain cells of grains indicated involvement of DAO in the supply of H2O2 for CPX mediated lignification process. Higher build up of thiobarbituric acid reactive substances, especially in cultivars PBW 343 (45 m mol g^-1 f.wt.) and PBW 550 (50 m mol g^-1 f.wt.) indicated their membrane instability during stress. Results revealed that both stresses caused substantial induction of coniferyl peroxidases (3.4-5.9 m mol min^-1 g^-1 f.wt.) to facilitate lignification. Differential genotypic response was observed under two stresses, C 306 and HD 2967 were resistant to heat stress, while PBW 175 and PBW 621 to drought stress.? ??

 Key words

Coniferyl alcohol peroxidase, Guaiacol peroxidase, Lignin, Syringaldazine peroxidase

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